The Protective Effects of 16, 16 Dimethyl PGE 2 Against CC1 4 Metabolism and Diet-Induced Hepatic Damage
Van Schoick, Timothy S.
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The effects of 16.16 dimethyl PGE2 on the expiration of CC14 was examined. Rats were given 100 ug/kg/ml 16.16 dimethyl PGE 2 or vehicle by subcutaneous injection 24. 18, and 0.5 hrs. before challenge with 2 ml/kg or 1 ml/kg 14CC1 4 , Expired air was collected and analyzed for 14CC1 4 . Significant differences were found between prostaglandin and vehicle treated groups at 2 ml/kg insult but not at 1 ml/kg. Serum levels of quality control rats indicated that prostaglandin protection was realized at 1 ml/kg challenge but not at 2 ml/kg. Thus. it appears that 16,16 dimethyl PGE 2 can protect the liver against CC1 4-induced necrosis without increased expiration of unmetabolized CC1 4. The protective effects of 16,16 dimethyl PGE 2 on rats given a nutrient deficient diet was also investigated. Rats were divided into four groups according to weight and fed a choline, folic acid, methionine, and vitamin B12 deficient diet! ethanol. Subcutaneous prostaglandin(25 ug/kg/ml) or vehicle treatment was administered twice daily. At the end of four weeks, ten rats from each group were sacrificed by decapitation. Serum and liver samples were collected and analyzed. No differences in serum parameters or histological evaluations were observed between prostaglandin and vehicle treated groups, Thus, at this dose(25 ug/kg/ml), 16,16 dimethyl PGE2 does not appear to offer protection against diet deficient-induced liver damage.