Cytotoxicity of Various Mammalian Cancer Cell Lines to Hyperthermia and Cell-Cycle Phase Sensitivity
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I compared the temperature sensitivities (40 - 44ºC) of various mammalian cell lines. HeLa and CHO were less sensitive to temperature than P388 and L12lo; for example, to kill 90% of the cells, 50 minutes of exposure was needed at 43°C for P388 and L12l0 compared to 90 minutes for HeLa and CHO at the Same temperature. I also investigated the temperature sensitivity of cells in the stationary phase of growth as compared to cells in the exponential (or logarithmic) phase. The difference observed was not statistically significant. L12l0 cells growing in vivo as ascites were much more sensitive than cells grown in vitro; for example, a 30 minute exposure to 43ºC allowed 60% of the cells in vitro to survive compared to 1.6% survival rate of ascites cells. The sensitivities of different phases of the cell cycle to temperature was investigated using partially synchronized Ll2l0 cessl and synchronized CHO cells. The DNA synthesizing phase, (S - Phase), was more sensitive than the "G 1', 'G 2" or "M" phases.