In Vitro Adherence of Streptococcus sanguis and Streptococcus mutans to Hydroxyapatite Beads Pretreated with Postulated Adsorbance Inhibitors and Stimulators
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Dental caries can be defined as decay of teeth due to demineralization of the surface by organic acids produced by oral bacteria colonizing on the tooth surface. Information as to the mechanism by which oral bacteria adhere to teeth is important in caries prevention research. The work reported here describes an in vitro assay of bacterial adherence using beads of hydroxyapatite (HA). the main inorganic component of teeth, as the adsorbent, with pretreatments of various postulated inhibitors and stimulators of bacterial adherence. The adsorbance of Streptococcus mutans BHT and Streptococcus sanguis JC43 to HA beads was demonstrated through 3 H - labeled cell scintillation measurements which proved effective in quantitatively determining adherence. This investigation was developed to test the various postulated mechanisms of bacterial accumulation and adsorption to tooth surfaces and was a result of current studies on the development of feasible cariostatic agents for caries prevention.
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