The Use of DNAase I as a Means of Probing Chromatin Structure in the Region of an Induced Gene
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Authors
Bruder, Francine
Issue Date
1983
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
In these experiments an attempt was made to establish the conditions
necessary for use of the endonuclease, DNAase I, in the elucidation of the
chromatin structure of transcriptionally active genes in the rat C6 glioma
cell line. DNAase I preferentially attacks transcriptionally active
chromatin, and sites hypersensitive to this enzyme have been found to occur
in a number of different cell types. This study was undertaken as a result
of the suspected presence of a DNAase I hypersensitive site in glioma cell
chromatin.
Description
v, 41 p.
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License
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