The Use of DNAase I as a Means of Probing Chromatin Structure in the Region of an Induced Gene
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In these experiments an attempt was made to establish the conditions necessary for use of the endonuclease, DNAase I, in the elucidation of the chromatin structure of transcriptionally active genes in the rat C6 glioma cell line. DNAase I preferentially attacks transcriptionally active chromatin, and sites hypersensitive to this enzyme have been found to occur in a number of different cell types. This study was undertaken as a result of the suspected presence of a DNAase I hypersensitive site in glioma cell chromatin.