Tryptic Peptide Fragments of the Principal Curarimimetic Neurotoxin from the Venom of the Thailand Cobra, Naja Naja Siamensis
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Authors
Zusman, Neil
Issue Date
1980
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Neurotoxin-3 from the venom of the Thailand Cobra,
Naja naja siamensis, was reduced and S-carboxymethylated
prior to undergoing digestion by trypsin. The resulting
five major tryptic peptide fragments were partially
separated chromatographically and their ability to bind
to the acetylcholine receptor site (AchR) was examined.
Results based on a 30-min. time point seemed to indicate
that some of the peptides inhibit the binding of 125 I-α-
bungarotoxin to the AchR at concentrations in the range
of 10-6 - 10-4 M. One particular peptide, which is
thought to contain the majority of important residues
making up the "toxic loop," exhibited pronounced inhibition.
Results from a kinetic binding study indicated
that this peptide possibly exhibits inhibition at a concentration
in the range of 3. 46 X 10 -6 - 6 .91 X 10 -6 M.
Description
viii, 75 p.
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