Development of a Sensitive Method for the Analysis of Proteinases Present in a Single Embryo
Sakal, Kathleen M.
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The method we chose to develop is based on,the principle of measurement of histamine released by enzymatic hydrolysis of a 'suitable' synthetic substrate. ·(l.e. one which possesses the basic structural requirements of a substrate for the enzyme (proteinase) to be analyzed for). In this initial attempt in its development, we utllize N-Benzoyl-L-Tyroslne Histamine (NBLT-H1) as a substrate for the model enzyme, α-chymotrypsln.