The Effects of Pretreatment of Pericardial Tissue on Alkaline Phosphatase Hydrolytic Activity and Extractability
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Authors
Buffenn, Angela N.
Issue Date
1989
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Bioprosthetic valves frequently fail due to
pathological mineralization, a process that begins in cell
remnants that have undergone glutaraldehyde treatment. It
has been found that other pathological cardiovascular
calcification as well as mineralization in both skeletal
and dental tissue is, to a great extent, initiated in matrix
vesicles. Matrix vesicles are membrane-enclosed structures
containing high levels of the enzyme alkaline phosphatase
(AP). As a result, the enzyme AP is believed to play an
important role in the mineralization of cardiac valves. The
objectives of these studies were 1) to determine the effects
of freezing and freeze-drying pericardial tissue on AP
activity and extractability, 2) to determine the effect of
prolonged glutaraldehyde storage on AP activity and
extractability, 3) to determine if AP activity returned with
implantation, and 4) to obtain maximum AP hydrolytic
activity using the substrate para-nitrophenol phosphate
(PNPP). It was concluded that 1) freezing and freeze-drying
pericardial tissue did not produce changes in either AP
activity or extractability, 2) AP activity and extratability
were lost with prolonged glutaraldehyde storage, 3) AP
activity returned with implantation, and 4) maximum AP
activity was obtained when samples were placed in a shaking
water bath and vortexed during the addition of PNPP.
Missing pages 14-16, 18, and 20.
Missing pages 14-16, 18, and 20.
Description
vi, 35 p.
Citation
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License
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