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dc.contributor.advisorLevy, Robert J.
dc.contributor.advisorWebb, Catherine L.
dc.contributor.authorBuffenn, Angela N.
dc.date.accessioned2012-01-25T20:53:50Z
dc.date.available2012-01-25T20:53:50Z
dc.date.issued1989
dc.identifier.urihttp://hdl.handle.net/10920/24696
dc.descriptionvi, 35 p.en_US
dc.description.abstractBioprosthetic valves frequently fail due to pathological mineralization, a process that begins in cell remnants that have undergone glutaraldehyde treatment. It has been found that other pathological cardiovascular calcification as well as mineralization in both skeletal and dental tissue is, to a great extent, initiated in matrix vesicles. Matrix vesicles are membrane-enclosed structures containing high levels of the enzyme alkaline phosphatase (AP). As a result, the enzyme AP is believed to play an important role in the mineralization of cardiac valves. The objectives of these studies were 1) to determine the effects of freezing and freeze-drying pericardial tissue on AP activity and extractability, 2) to determine the effect of prolonged glutaraldehyde storage on AP activity and extractability, 3) to determine if AP activity returned with implantation, and 4) to obtain maximum AP hydrolytic activity using the substrate para-nitrophenol phosphate (PNPP). It was concluded that 1) freezing and freeze-drying pericardial tissue did not produce changes in either AP activity or extractability, 2) AP activity and extratability were lost with prolonged glutaraldehyde storage, 3) AP activity returned with implantation, and 4) maximum AP activity was obtained when samples were placed in a shaking water bath and vortexed during the addition of PNPP.en_US
dc.description.abstractMissing pages 14-16, 18, and 20.
dc.description.sponsorshipDepartment of Pediatric Cardiology. C.S. Mott Children's Hospital. Ann Arbor, Michigan.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.ispartofKalamazoo College Health Sciences Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Health Sciences;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleThe Effects of Pretreatment of Pericardial Tissue on Alkaline Phosphatase Hydrolytic Activity and Extractabilityen_US
dc.typeThesisen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis.


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