A Study of the Interferon Assay and the Effect of interferon and Tunicamycin on Cell Proliferation and Interleukin Production
Armbrustmacher, Paula Michelle
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Interferons (INF) are the body's first line of defense against viral infection. There are three groups of IFN which are induced and produced by different mechanisms and an assay has been developed to ascertain the amount and type of IFN necessary to protect cells infected with virus. LB cells were treated with murine IFN and allowed to incubate overnight. The cells were then challenged with vesicular stomatitis virus or encephalomyocarditis virus and incubated up to 48 hours. Cells were then treated with a formalin solution and stained with crystal violet to determine the titer of the original sample used. Original sample titers were then compared with and without a reference unit and the percentage of the titer obtained in terms of the labelled titer were calculated. Samples were found to have small percentages of IFN indicating a large decrease in sample potency. The highly reproducible sample runs indicated a consistent environment whereas the differing titers of interferons necessary to protect cells against virus indicates different sample strengths. Interleukins are important in the activation of lymphocytes and the body's immune response. An experiment was designed to investigate the effect of IFN and tunicamycin (TM) on interleukin 1 (IL-i) and interleukin 2 (IL-2) and cell proliferation. Mice were injected with IFN (20,000 U) and TM in various concentrations. Spleens were removed at 72 hours and at 96 hours and lymphocytes were isolated. Using three separate assays, cell proliferation and interleukin 1 and 2 production were determined. Cell proliferation seemed uninhibited by IFN at 72 hours and showed little variation with changing TM dosage. At 96 hours cell proliferation showed inhibition with IFN and with TM + IFN. Data for interleukin production are unavailable though it is believed that TM would inhibit IL-l and IL-2 production while IFN would have either little to no effect on either. The addition of IFN to cells may compensate for the inhibition of IL-l and IL-2 by TM.
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