Interaction of Human Lymphocytes and Mouse Spleen Cells with Wheat Germ Mitogen
Baughman, Andrew L. (Drew)
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The immune response is a complex phenomenon; certain mechanisms initiate the production of antibodies and certain others arrest it from unlimited activation of lymphocytes. The "suppressor" mechanisms are unknown. Lectins, in particular mitogens such as concanavalin A derived from the jack bean and wheat germ mitogen extracted from wheat germ, aid investigation of suppression., In this investigation, wheat germ mitogen and potato lectin were isolated and used to investigate suppression of human lymphocytes. Enhancement, and not suppression, was observed in the controls of the experiments. Other data may indicate that the manner in which WGM activated human lymphocytes specifically suppress WGM induced proliferation is through an N-acetyl-D-glucosamine specific molecule such as WGM itself or potato lectin. WGM induced stimulation of mouse spleen cells has been observed; this investigation includes attempts to determine the optimum conditions for stimulation. The best stimulation was produced when 2.5 x 10 6 C57 mouse spleen cells were cultured in Clicks medium with 5-10 ug WGM.