The Characterization of Two Antibiotic Resistance Plasmids in Enterobacteriaceae
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Authors
Gilmore, Michael S.
Issue Date
1977
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The purpose of this research was two-fold. Plasmids, discovered
in two clinical isolates obtained from Bronson Meth0dist
Hospital in Kalamazoo, were first screened for novel modes of antibiotic
inactivation. Preliminary studies performed at Bronson indicated
that the Gentamicin-Tobramycin resistance levels were inversely
related, but it was uncertain whether this relationship was attributable
to physiological differences (one strain being Klebsiella sp.
and the other E. coli, UC6356 and UC6357 respectively), or actual differences
in the plasmid encoded antibiotic inactivating enzymes.
The second reason for conducting this research was to characterize
the bands produced by gel electrophoretic analysis of the restriction
endonuclease digested plasmid fragments with respect to antibiotic
resistance, and to assess the value of each for future use as vehicles
for the transformation of foreign DNA into bacteria.
Description
ii, 39 p.
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License
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