The Characterization of Two Antibiotic Resistance Plasmids in Enterobacteriaceae
Gilmore, Michael S.
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The purpose of this research was two-fold. Plasmids, discovered in two clinical isolates obtained from Bronson Meth0dist Hospital in Kalamazoo, were first screened for novel modes of antibiotic inactivation. Preliminary studies performed at Bronson indicated that the Gentamicin-Tobramycin resistance levels were inversely related, but it was uncertain whether this relationship was attributable to physiological differences (one strain being Klebsiella sp. and the other E. coli, UC6356 and UC6357 respectively), or actual differences in the plasmid encoded antibiotic inactivating enzymes. The second reason for conducting this research was to characterize the bands produced by gel electrophoretic analysis of the restriction endonuclease digested plasmid fragments with respect to antibiotic resistance, and to assess the value of each for future use as vehicles for the transformation of foreign DNA into bacteria.