Investigations into DNA Damage Induced by Alkylating Agents and Ultraviolet Radiation in Chinese Hamster Cells
Ryan, Randall E.
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This study investigates the correlations that exist between DNA damage, DNA repair, mutagenesis and carcinogenesis, focusing on the damage aspect. The alkylating agents MNNG, MNU and MMS and ultraviolet radiation were evaluated for their ability to induce chromosomal damage, using an in vitro DNA damage/alkaline elution assay, following exposure to V-79 Chinese hamster cells. A procedure for making the standard elution assay more sensitive by decreasing its rate of elution was also developed. Dose dependent damage was detected for all agents with the modified slow-assay demonstrating increased elution of DNA to that of the standard assay.