Selected Studies on the Evaluation of DNA Damage in Chinese Hamster Cells Induced by Metal Ions
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Authors
McVey, John E.
Issue Date
1977
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
There is documented evidence that certain metals and metal ions
are carcinogenic. Pb, As, Ni, Cu, Cd, Be and Se have been shown to
cause tumors under specific conditions in experimental animals. This
investigation was undertaken in an attempt to observe the extent of
chromosomal damage induced by various metals and metal ions. An in
vitro DNA damage/alkaline elution assay (Kohn, et al., 1974) utilizing
Chinese hamster V-79 cells, with or without a liver microsomal activation
system, was employed in order to detect the induced chromosomal
damage. Extensive damage was observed in V-79 cell preparations
treated with Na2 Se03, Na2 Se04' Pb(C2H302)2 and Na As02. CuS04,
BeS04 and Cd(N03)2 also induced DNA damage but at toxic levels. Three
noncarcinogens, Cr03, KCl and MgC12, did not cause increases in the
elution pattern. In the presence of the activation system, Na2 Se03
and Na2 Se04 induced detectable DNA damage at lower concentrations.
Description
vi, 36 p.
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