Selected Studies on the Evaluation of DNA Damage in Chinese Hamster Cells Induced by Metal Ions
McVey, John E.
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There is documented evidence that certain metals and metal ions are carcinogenic. Pb, As, Ni, Cu, Cd, Be and Se have been shown to cause tumors under specific conditions in experimental animals. This investigation was undertaken in an attempt to observe the extent of chromosomal damage induced by various metals and metal ions. An in vitro DNA damage/alkaline elution assay (Kohn, et al., 1974) utilizing Chinese hamster V-79 cells, with or without a liver microsomal activation system, was employed in order to detect the induced chromosomal damage. Extensive damage was observed in V-79 cell preparations treated with Na2 Se03, Na2 Se04' Pb(C2H302)2 and Na As02. CuS04, BeS04 and Cd(N03)2 also induced DNA damage but at toxic levels. Three noncarcinogens, Cr03, KCl and MgC12, did not cause increases in the elution pattern. In the presence of the activation system, Na2 Se03 and Na2 Se04 induced detectable DNA damage at lower concentrations.