A Study of the Role of Glycoprotein gX in Pseudorabies Virus Replication in African Green Monkey Cells
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Pseudorabies virus (PRV) is an alphaherpes virus that infects and kills a wide range of animals. The main host is swine. It has some homology with herpes simplex virus (HSV). PRV produces at least seven glycoproteins, of which glycoprotein gX is one. Glycoprotein gX, which is secreted into the medium, appears in all strains of wild type PRY (Ben-Porat et aI., 1970). The function of gX was studied by comparing a wild type PRV with gX mutant PRV. These two viruses were mixed at various ratios of multiplicity of infection (moi) (for example 1:1, 0.1:1 etc.) and were used to infect African Green Monkey (vero) cells. Titers of the mix-infected cells was determined using the black plaque assay. This was the first passage. A fresh set of vero cells were infected with the infected cells of the first passage-which constituted the second passage. This same procedure was repeated to produce the third passage. The purpose of passing the mix-infected cells to fresh vero cells was to determine whether either virus exhibited a selective advantage over the other. The selective advantage would result in the use of gX as an alternative candidate to prepare a live vaccine. In addition mice were infected with wild-type virus and gX mutant virus. Their brain tissue was then examined for the presence of gX mutant virus. Black plaque was done and it was observed that both wild-type virus and gX mutant virus were present. An important replication difference between the two viruses was not observed.