A Study of the Role of Glycoprotein gX in Pseudorabies Virus Replication in African Green Monkey Cells
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Authors
Demissie, Sossiena
Issue Date
1988
Type
Thesis
Language
en_US
Keywords
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Abstract
Pseudorabies virus (PRV) is an alphaherpes virus that infects
and kills a wide range of animals. The main host is swine. It has
some homology with herpes simplex virus (HSV). PRV produces at
least seven glycoproteins, of which glycoprotein gX is one.
Glycoprotein gX, which is secreted into the medium, appears in all
strains of wild type PRY (Ben-Porat et aI., 1970). The function of gX
was studied by comparing a wild type PRV with gX mutant PRV.
These two viruses were mixed at various ratios of multiplicity of
infection (moi) (for example 1:1, 0.1:1 etc.) and were used to infect
African Green Monkey (vero) cells. Titers of the mix-infected cells
was determined using the black plaque assay. This was the first
passage. A fresh set of vero cells were infected with the infected
cells of the first passage-which constituted the second passage. This
same procedure was repeated to produce the third passage. The
purpose of passing the mix-infected cells to fresh vero cells was to
determine whether either virus exhibited a selective advantage over
the other. The selective advantage would result in the use of gX as
an alternative candidate to prepare a live vaccine. In addition mice
were infected with wild-type virus and gX mutant virus. Their brain
tissue was then examined for the presence of gX mutant virus. Black
plaque was done and it was observed that both wild-type virus and
gX mutant virus were present. An important replication difference
between the two viruses was not observed.
Description
III, 33 P.
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