TCR Genotyping of T Cell Hybridomas Derived from Lymphocytic Infiltrates of Mammary Tumors in BALB/C Mice
Robichaud, Nicole MacLean
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Before the development of successful immunotherapeutic regimens is possible to combat breast cancer, a better understanding of the lymphocytes infiltrated in the tumors is needed. If it could be proven that the lymphocytic infiltrates are oligoclonal in nature, knowing the characteristics of the 90riginally infiltrating T cells could possibly reveal much about the organism's reaction to the tumor cell antigen. T cell hybridoma lines were used for investigation. These were formed by the fusion of T cells, derived from lymphocytic infiltrates, with a BW5147 thymoma cells (Hendrick, S. et al., 1982). Genotypic analysis of the beta region of the T cell receptor genetic locus was conducted. Common patterns of genetic rearrangement were investigated using radiolabelled probes specific for certain areas of the locus. Of the T cell hybridoma lines examined, only one showed evidence of a genetic rearrangement within the TCR locus. Data obtained from FACS analysis reinforced this finding. The rearrangement was mapped to a region beyond J-beta 1.2. The other cell lines showed no genetic rearrangement, only germ-line bands and fragments. The FACS data failed to give any further insight into these findings. These inconclusive results have brought up the issue of possible problems in the procedures for culturing the tumor infiltrates, and hybridoma formation. with changes made in these methods, perhaps further investigation of . the TCR locus can continue with greater success.