Phenotypic and Genotypic Changes in U-77779-Resistant Chinese Hamster Lung (V79) Cells
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Authors
Butryn, Robert Kevin
Issue Date
1990
Type
Thesis
Language
en_US
Keywords
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Abstract
Tumor cells have been shown to develop drug-resistance in a large
number of cancers. The resistant portion of tumor cells is important in the
treatment of cancer because it is less sensitive to chemotherapy. The
purpose of this project was to identify V79 cells which had been made
resistant to the alkylating agent U-77779, and to characterize them in
terms of cross-resistance to other anti-tumor drugs, cell cycle progression,
Glutathione (GSH) levels, and mdrl-mRNA levels. The V79 cells made
resistant to U-77779 showed the multi-drug resistant phenotype which
included a reduced sensitivity to a variety of anti-tumor drugs. Cell cycle
progression analysis by flow cytometry revealed an absence of polyploid
cells in the resistant line. The resistant line was also found to have
increased mdrl-mRNA levels. The resistant line did not, however, show
increased levels of GSH.Tumor cells have been shown to develop drug-resistance in a large
number of cancers. The resistant portion of tumor cells is important in the
treatment of cancer because it is less sensitive to chemotherapy. The
purpose of this project was to identify V79 cells which had been made
resistant to the alkylating agent U-77779, and to characterize them in
terms of cross-resistance to other anti-tumor drugs, cell cycle progression,
Glutathione (GSH) levels, and mdrl-mRNA levels. The V79 cells made
resistant to U-77779 showed the multi-drug resistant phenotype which
included a reduced sensitivity to a variety of anti-tumor drugs. Cell cycle
progression analysis by flow cytometry revealed an absence of polyploid
cells in the resistant line. The resistant line was also found to have
increased mdrl-mRNA levels. The resistant line did not, however, show
increased levels of GSH.
Description
vi, 63 p.
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