Phenotypic and Genotypic Changes in U-77779-Resistant Chinese Hamster Lung (V79) Cells

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Authors
Butryn, Robert Kevin
Issue Date
1990
Type
Thesis
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en_US
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Abstract
Tumor cells have been shown to develop drug-resistance in a large number of cancers. The resistant portion of tumor cells is important in the treatment of cancer because it is less sensitive to chemotherapy. The purpose of this project was to identify V79 cells which had been made resistant to the alkylating agent U-77779, and to characterize them in terms of cross-resistance to other anti-tumor drugs, cell cycle progression, Glutathione (GSH) levels, and mdrl-mRNA levels. The V79 cells made resistant to U-77779 showed the multi-drug resistant phenotype which included a reduced sensitivity to a variety of anti-tumor drugs. Cell cycle progression analysis by flow cytometry revealed an absence of polyploid cells in the resistant line. The resistant line was also found to have increased mdrl-mRNA levels. The resistant line did not, however, show increased levels of GSH.Tumor cells have been shown to develop drug-resistance in a large number of cancers. The resistant portion of tumor cells is important in the treatment of cancer because it is less sensitive to chemotherapy. The purpose of this project was to identify V79 cells which had been made resistant to the alkylating agent U-77779, and to characterize them in terms of cross-resistance to other anti-tumor drugs, cell cycle progression, Glutathione (GSH) levels, and mdrl-mRNA levels. The V79 cells made resistant to U-77779 showed the multi-drug resistant phenotype which included a reduced sensitivity to a variety of anti-tumor drugs. Cell cycle progression analysis by flow cytometry revealed an absence of polyploid cells in the resistant line. The resistant line was also found to have increased mdrl-mRNA levels. The resistant line did not, however, show increased levels of GSH.
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vi, 63 p.
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