Restriction Endonuclease Maps of the Pseudorabies Virus, Rice Strain, Genome
Long, Graham W.
MetadataShow full item record
Pseudorabies virus (PRV) is a herpesvirus that attacks most mammals and has caused serious economic problems in the swine industry. Since available vaccines have not been efficacious in controlling this virus, more information about its molecular biology must be elucidated in order to control it. One fundamental tool used in this research process is the restriction endonuclease map. Restriction endonucleases are enzymes that recognize certain base sequences along the DNA double helix and cleave it at these sites. A restriction map, then, is a diagram of the locations of the recognition sites of certain restriction enzymes along the DNA molecule and their geographic relation to each other. Using genomic Rice strain PRV DNA and a set of plasmids containing PRV BamHI fragments, restriction maps were constructed for EcoRV, HindIII, BglII, BamHI, KpnI, and HpaI, using gel electrophoresis and Southern blot techniques. With these maps, the locus of the PRV major capsid protein gene was determined. This, along with another previously mapped gene and the herpes simplex virus (HSV) gene map, was then used to determine the non-colinearity of the PRV and HSV genomes. As a result, the loci of the genes along the HSV genome can not be used in determining the loci of these same genes along the the PRV genome.