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dc.contributor.advisorYeramilli, Venkata
dc.contributor.authorPaul, Caitlin
dc.descriptionv, 27 p.en_US
dc.description.abstractThe preimmune antibody repertoire plays an important role in immune protection through out the life of the organism. In rabbits, B cells develop in the bone marrow and migrate to the gut associated lymphoid tissues (GALT), where in the presence of commensal bacteria, they are stimulated to proliferate, form organized follicles and develop -the primary antibody repertoire by Ig gene diversification. While the molecular events leading to Ig gene diversification in the bone marrow are well understood, little is known about the mechanisms regulating B cell proliferation and homeostasis in GALT. In a previous study to test whether B cell activating factor (BAFF) may play a role in stimulating GALT B cells, endogenous BAFF was neutralized in vivo using a soluble decoy receptor (TACI-Ig). This treatment greatly reduced the number and size of proliferating B cell follicles, demonstrating that BAFF is required for B cell development in GALT. However, it was not clear from this study, whether BAFF provided a proliferation signal or survival/maintenance signal. Therefore to address this question, I cloned, expressed and purified recombinant rabbit BAFF from E. coli, and tested its affect on B cells in vitro with a [3H]-thymidine incorporation assay. Purified rabbit BAFF induced splenocytes to undergo proliferation, but not B cells isolated from GALT. However, addition of BAFF to cultures of GALT B cells transiently prolonged their survival, suggesting that BAFF may provide a survival/maintenance signal for B cells in GALT.en_US
dc.description.sponsorshipDr. Katherine L. Knight Lab. Department of Microbiology and Immunology. Stritch School of Medicine. Loyola University Chicago. Maywood, Illinois.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleCloning, expression and activity of B cell Activating Factoren_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Integrated Projects [1649]
    This collection includes Senior Integrated Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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