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dc.contributor.advisorNaik, Jay
dc.contributor.authorWestphal, Joslyn
dc.date.accessioned2011-12-05T19:02:20Z
dc.date.available2011-12-05T19:02:20Z
dc.date.issued2008
dc.identifier.urihttp://hdl.handle.net/10920/24219
dc.descriptioniv, 23 p.en_US
dc.description.abstractNanotechnology, the manipulation and manufacturing of particles less than 100 nm in size, is at the forefront of scientific research. However, there is evidence that the size-dependent properties of nanoparticles give rise to high toxicity levels. Studies have shown that exposure to inhaled nanoparticles, including titanium dioxide (Ti02), is associated with increased reactive oxygen species (ROS), leading to endothelial dysfunction. To date, most studies on nanoparticle toxicity are based on the effects on cells after particles have been internalized. However, we hypothesized that internalization is not necessary to cause dysfunction. To test our hypothesis, we examined changes in endothelial dilation in response to a vasodilator, ionomycin. We expected percent vasodilation to be lower in groups treated with Ti02, indicating dysfunction. Particle exposure was accomplished using two separate doses ofTi02 (0.05% v/v, 0.1 % v Iv) using the isolated perfused lung preparation of 17 rat lungs. Lungs were exposed for 1 hour, a time period over which we expected no internalization of Ti02. We observed an increase in pulmonary arterial resistance after the addition ofTi02 to the perfusate, which we can attribute to an increase in shear stress. We found a significant increase in percent dilation with increasing amounts of ionomycin; however, our data showed no significant difference in percent dilation between groups at each individual dose of ionomycin. Therefore, our initial hypothesis was not supported, and we concluded that internalization of Ti02 may indeed be necessary to cause pulmonary endothelial dysfunction. Future studies should analyze the localization of Ti02 within the endothelial cells to conclusively determine whether the particles were internalized or not, and should also examine the effects of other nanoparticles on endothelial function.en_US
dc.description.sponsorshipDepartment of Biology. New Mexico Institute of Mining and Technology. Socorro, New Mexico.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleEffects of short-term exposure to titanium dioxide on pulmonary endothelial functionen_US
dc.typeThesisen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis.


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  • Biology Senior Individualized Projects [1489]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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