Replication of Tanapox Virus in Owl .Monkey Kidney Cells and Human Glioblastoma-Astrocytoma Cells
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Authors
Sket, Georgina M.
Issue Date
2010
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Tanapox virus (TPV) is a human and primate pathogen that causes relatively mild,
self-limiting febrile illness. It is a member of the genus Yatapoxviridae in the
poxvirus family. The virus only replicates in human and primate cells. It is speculated
that TPV infection is spread through a biting arthropod vector. Green fluorescent
protein (GFP) is a chemoluminescent protein that can be incorporated into virus DNA
and serve as a marker for infection. A recombinant version of Tanapox virus (TPVGFP)
is frequently used in studies involving TPV. This study aims to examine
whether the incorporation of the GFP gene into the TPV DNA negatively affects
virus replication rate within ~ts host. Furthermore, this study will observe replication
rates at 35°C and at 37°C in order to corroborate claims that the virus also grows at
temperatures lower than primate physiological temperature. It is hypothesized that, in
keeping with previous claims, temperature will not affect the virus replication rate. It
is also hypothesized that there will not be a difference in replication rates between
TPV-CDC and TPV-GFP. In this experiment, TPV-GFP and TPV-CDC wer~ grown
in Owl Monkey Kidney (OMK) cells and Human Glioblastoma-Astrocytoma (U373)
cells. A virus titer was then performed using OMK cells. Using ANOVA there was
found to be significant variation within the means, and Student's t-test found the
differences between the means ofTPV-CDC grown in OMK cells at 37°C and TPVCDC
grown in U373 cells at 35°C to be significantly different from other means at
the p > 0.05 level. Therefore, while the hypothesis that temperature does not affect
replication was proven, our study claims that the inclusion of GFP into the viral
genome negatively affects the overall replication rate of the virus.
Description
v, 33 p.
Citation
Publisher
Kalamazoo College
License
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