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dc.contributor.advisorPezzuto, John
dc.contributor.authorAppledorn, Daniel M.
dc.date.accessioned2011-10-26T12:59:29Z
dc.date.available2011-10-26T12:59:29Z
dc.date.issued2000
dc.identifier.urihttp://hdl.handle.net/10920/23809
dc.descriptioniv, 32 p.en_US
dc.description.abstractExcessive production of nitric oxide and cyclooxygenase 2 (COX-2) are thought to be causative factors of cellular injury and cancer. Sulforaphane acts as a potent detoxification enzyme inducer. We tested sulforaphane and oxomate for inhibition of inducible nitric oxide synthase (iNOS) and COX-2 in cultured mouse macrophage cells. Western blot analysis of whole cell lysates revealed that sulforaphane mediated a dosedependent decrease of lipopolysaccharide (LPS)-stimulated iNOS and COX-2 expression. Application of oxomate to LPS-stimulated macrophage cells revealed dosedependent inhibition of iNOS expression while no inhibition of COX-2 was detected. In mouse skin models, we found 12-0-Tetradecanoyl-phorbol-13-acetate (TPA) promoted oxidative stress events~ including altered levels of the reduced glutathione (GSH), myeloperoxidase and superoxide dismutase, that correlate with cancer. Results show unaltered levels of GSH in mice pretreated with sulforaphane and oxomate. These findings suggest that sulforaphane and oxomate have the potential to serve as cancer chemopreventive agents.en_US
dc.description.sponsorshipDepartment of Pharmaceutics and Pharmacodynamics. University of Illinois at Chicago. Chicago, Illinois.
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleSULFORAPHANE AND OXOMATE: INHIBITORS OF TPA INDUCED NITRIC OXIDE SYNTHASE (INOS), CYCLOOXYGENASE 2 (COX-2) AND OXIDATIVE STRESS IN MURINE MACROPHAGE AND SKIN MODELSen_US
dc.typeThesisen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis.


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    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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