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dc.contributor.advisorOlivero, Ofelia
dc.contributor.authorPietras, Sara
dc.descriptionvi, 26 p.en_US
dc.description.abstractIn 1987 the first anti-AIDs drug, 3'-azido-2', 3'-dideoxythymidine (AZT), was approved. Today AZT treatment includes persons with human immunodeficiency virus type 1 (HIV -1), of all ages and at all stages of the disease. One AZT treatment group is pregnant women, as AZT has been shown to significantly reduce the chance of vertical HIV -1 transmission. However AZT has recently been shown to produce tumors in the offspring of mice who are treated with it during the last third of pregnancy. In these studies, we assessed the incorporation of AZT into the genome of human lymphoblastoid (TK6) cells exposed to the drug for increasing amounts of time, and also in dose increments. Furthermore, we examined the role of AZT in DNA methylation. DNA was extracted with a non-organic extraction kit or phenol-chloroform technique. Genomic incorporation was determined via anti-AZT radioimmunoassay. Methylation was examined using restriction enzymes. F or cells exposed to AZT for increasing times, we found increasing genomic incorporation, but inconclusive methylation results. For the cells that were treated with increasing concentrations of AZT, we found more incorporation at the highest concentration. The methylation study of these cells showed a positive correlation between methylation and AZT incorporation. More AZT is thus incorporated into DNA with increased dosage and longer exposure. With increasing incorporation, AZT also induces increasing methylation. Careful examination of these effects in consideration of human treatments would seem appropriate.en_US
dc.description.sponsorshipNational Cancer Institute. National Institutes of Health. Bethesda, Maryland.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleThe Effects of Dosage and Exposure Time on the Genomic Incorporation of 3'- Azido-2', 3'-Dideoxythymidine (AZT) in TK6 Cells, and the Role of AZT in DNA Methylationen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Individualized Projects [1550]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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