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dc.contributor.advisorStapleton, Susan
dc.contributor.authorNelson, Joshua N.
dc.descriptioniv, 21 p.en_US
dc.description.abstractDuring the past several years, the ability of certain elements to act in the capacity of insulin-mimetics in diabetic and other therapies has drawn significant interest. The purpose of this study was to observe the effects of toxic metals such as cadmium on the activity of glucose-6-phosphate dehydrogenase (G6PDH) so as to create a comparison with medicinal metals such as zinc. G6PDH acts as a rate-limiting enzyme in the pentose phosphate pathway, a major pathway necessary for both carbohydrate and fatty acid metabolism and maintenance of the redox state of the cell. For our purposes, a stable cell line of Buffalo Rat Liver (BRL) cells was treated with varying concentrations of cadmium. Endogenous G6PDH activity showed a slight increase at 1.0 µM [Cd2+], while G6PDH promoter activity showed almost a two-fold increase that surpassed the effects of insulin. These results indicate that cadmium, at certain levels, is capable of regulating G6PDH activity. However, the results do not conclude the method by which this regulation occurs.en_US
dc.description.sponsorshipDepartment of Chemistry. Western Michigan University. Kalamazoo, Michigan.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleThe Effects of Cadmium on Glucose-6-Phosphate Dehydrogenase Activity in Buffalo Rat Liver Cellsen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Individualized Projects [1549]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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