Genetic Variation among Individuals of Batrachospermum helminthosum from Spring Brook, Kalamazoo, Michigan
Filkin, Nanda R.
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A study was conducted which examined genetic relatedness among 21 individuals of Batrachospermum helminthosum from Spring Brook, a stream in Kalamazoo, Michigan. The molecular marker technique, ISSR (Inter-Simple Sequence Repeats), was employed. This technique is new and has been used only once before to understand variation in algae. ISSR is a form of PCR (Polymerase Chain Reaction) in which DNA is replicated between specific micro satellite loci. These specific loci are determined by the primers used. In this study, six ISSR primers were used- ISSR 4, 8, 9, 10, 11 and 12. Gel electrophoresis was used to separate the replicated portions of DNA into bands. Bands were scored as 1 if present and 0 if not for each individual. This raw data (1 's and O's) was examined using PHYLIP (Phylogeny Inference Package) and a UPGMA tree showing relative relatedness among individuals was created. Relative relatedness within the stream was explained by controlled gene flow within mini populations. Overall, little gene flow occurred and within this population, minipopulations with related genomes were maintained within small clusters.