JavaScript is disabled for your browser. Some features of this site may not work without it.
  • About K
  • Academics
  • Admission
  • Alumni Relations
  • Giving to K
  • News & Events
  • Student Life
  • HORNET HIVE
  • ATHLETICS
  • SITEMAP
  • WEBMAIL
    • Login
    View Item 
    •   CACHE Homepage
    • Academic Departments, Programs, and SIPs
    • Biology
    • Biology Senior Integrated Projects
    • View Item
    •   CACHE Homepage
    • Academic Departments, Programs, and SIPs
    • Biology
    • Biology Senior Integrated Projects
    • View Item

    The Baculovirus Expression Vector Produces a Single Isoelectric Variant of Galectin-3

    Thumbnail
    View/Open
    Searchable PDF / Kalamazoo College Only (2.942Mb)
    Date
    1996
    Author
    Sandro, Luke
    Metadata
    Show full item record
    Abstract
    Galectin-3, an animal lectin known to be involved in pre-mRNA splicing (Agrwal et aI, 1993), has been found in phosphorylated and unphosphorylated forms. The unphosphorylated form occurs mainly in the nucleus, while the phosphorylated form is present mainly in the cytoplasm. Correspondingly, quiescent cells contain predominantly the phosphorylated, cytoplasmic form, while proliferating cells have mainly the unphosphorylated nuclear form. A bacterial expression vector has been engineered and proven to produce solely the unphosphorylated form (Agrwal et aI, 1993). In this study, a recently constructed baculovirus expression vector for galectin-3 (Voss, unpublished) was used to produce and isolate the protein, which was then analyzed by two-dimensional electrophoresis. Only one form of gal-3 was observed, and it is hoped that the eucaryotic machinery of the infected insect cells has resulted in a vector which produces solely the phosphorylated form. However, attempts to confirm this by dephosphorylation with alkaline phosphatase have been as yet inconclusive.
    URI
    http://hdl.handle.net/10920/23669
    Collections
    • Biology Senior Integrated Projects [1614]

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    DSpace software copyright © 2002-2022  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV
    Logo

    Kalamazoo College
    1200 Academy Street
    Kalamazoo Michigan 49006-3295
    USA
    Info 269-337-7000
    Admission 1-800-253-3602

    About K
    Academics
    Admission
    Alumni Relations
    Giving to K
    News & Events
    Student Life
    Sitemap
    Map & Directions
    Contacts
    Directories
    Nondiscrimination Policy
    Consumer Information
    Official disclaimer
    Search this site


    Academic Calendars
    Apply
    Bookstore
    Crisis Response
    Employment
    Library
    Registrar
    DSpace Express is a service operated by 
    Atmire NV