Studies on the Expressions of Caenorhabditis elegans K+ Channels and nACh Receptors in Xenopus oocytes
Loading...
Authors
Okuniewski, Diana E.
Issue Date
2001
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The effectiveness of drugs used to treat diseases caused by gastrointestinal
nematodes has diminished due to the proliferation of strains resistant to the currently
marketed anthelmintics. Oxindole alkaloid compounds (paraherquamide/marcfortine
family) however, are one of the new classes of anthelmintic reported since the
introduction of the macrocyclic lactones. These drugs are broad-spectrum anthelmintics
that even affect many drug-resistant strains of nematodes (Zinser, 2001). Our study was
designed to investigate the mechanism of action of oxindole alkaloid compounds using
the Xenopus oocyte system. We used the oocyte system to express a nicotinic
acetylcholine receptor (nAChR), unc-38, which we cloned from the free-living nematode,
Caenorhabditis elegans. In order to facilitate expression of this receptor, we also
included perfusion with PNU-141962 (2-deoxoparaherquamide) in an effort to study the
drug's phannacology.
Secondly, we tested the six amino acid modification, FCYENE (export signal), to
determine if it is effective in increasing expression of CeK+ channels in oocytes, and to
help determine if this segment can be used to increase expression of any type of gene.
Minimal expression was observed of the CeKl channel by itself. Additionally, the
CeK1 + FCYENE modification did not improve CeK1 expression, suggesting that
FCYENE may not be a useful export signal in the Xenopus oocyte system for nematodal
receptors even though it has been found to enhance expression for mammalian receptors (Ma et al., 2001).
Description
vii, 67 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.