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dc.contributor.advisorLeach, Karen L.
dc.contributor.authorMandrekar, Shweta
dc.descriptioniv, 37 p.en_US
dc.description.abstractCurrent research in pharmaceuticals is primarily focused on cell signaling pathways. G-proteins (GTP binding proteins) are a particularly favorite drug target because of their ubiquitous expression and the vast number of agonists that bind to them. Due to these factors they are implicated in a whole array of physiological diseases and disorders. SEQ-9, a novel G-protein coupled receptor (GPCR), was identified from a proprietary human genomic database and is characterized by common motifs that are shared among GPCRs. The goal of the project was to clone a novel full-length GPCR ID'd from a proprietary database. The function of the resulting construct was characterized by assays commonly used to study GPCR function in cell signaling pathways. A BLAST search indicated that SEQ-9 was the human homolog of the rat EDG-8, sharing a 87.7% identity at the amino acid level. Edg-8 has been shown to have SIP (Spingosine-I-Phosphate) as its ligand therefore, this information raised the possibility of SIP as a ligand for SEQ-9. An adenylyl cyclase assay showed that SIP induced an inhibition of forskolin-stimulated intracellular cyclic AMP levels suggesting coupling through a G.-coupled signaling pathway. A FLIPR () assay was also performed to monitor levels of calcium within the cells. A four to five-fold increase in calcium mobilization was seen when the cells were stimulated with SIP, suggesting coupling through a Gq signaling pathway. These experiments strongly suggest that SIP is a ligand for SEQ-9, and that this GPCR couples through both the Gi and Gq signaling pathways.en_US
dc.description.sponsorshipDepartment of Cellular and Molecular Biology. Pharmacia, Inc. Kalamazoo, Michigan.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleCharacterization of SEQ-9 an Orphan G protein-Coupled Receptoren_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Individualized Projects [1405]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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