Bone Effects of Hyperparathyroidism in Vitamin D Deficient Patients
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This study focused on the tendency for vitamin D deficient patients to develop bone marrow fibrosis, and parathyroid hormone's possible role in this process. Patients at the Henry Ford Hospital Bone and Mineral Clinic were selected based on selected variables, with bone biopsy, stain type and vitamin D deficiency levels being the priorities. The total pool of patients consisted of 19 individuals, all of whom had a bone biopsy done within the past 5 years at Henry Ford Hospital and had detailed biochemical studies. Biochemical data from past assays were reviewed to determine chemical levels of calcium, magnesium, creatinine, alkaline phosphate, albumin, globulin, phosphate, intact parathyroid hormone, and BSAP levels at the time of the patient's biopsy. Bone biopsy samples were stained using Goldner's trichrome staining technique and measurements were made utilizing the bioquant system. Determinations were made whether a patients bone biopsy had bone marrow fibrosis or not by three experienced lab personnel, and the patients were separated into groups depending upon their decision. Measured values were evaluated depending upon the significance in the difference between the two groups. However, due to the surprisingly small sample that tested positive for bone fibrosis, a traditional T-test proved to be inaccurate. The data at first appeared to show that PTH, along with alkaline phosphatase, corresponded with the development of fibrosis. The tissue measurements made further support these findings, as differences in bone erosion coincided with the biochemical data. However, the T-test performed upon the data showed these differences in mean to most probably be due to statistical error. Due to the difference in sample sizes and the large variance, the data has proven to be inconclusive. It has, however, provided a starting point for future study.