Determination of the location and intensity of the SPD-collectin in Black 6 and Balb/c types of mice infected with Pseudomonas aeruginosa
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When eyes of mice become infected with Pseudomonas aeruginosa, the surfactant protein D (SPD)- collectin, which helps in the innate host defense against various bacterial, fungal, and viral pathogens in pulmonary infections, is up regulated in the epithelium of the cornea 1, 3, 5, and 7 days post infection. Mice were infected with Pseudomonas aeruginosa by scarifying the left cornea with three I-mm incisions and then topically applying Pseudomonas aeruginosa. The eyes were then enucleated (taken from the mice whole) and frozen using Tissue TEK and liquid nitrogen. From the frozen blocks, cross-sections of the eye were taken and immunostained for the SPD-collectin. Both the Black 6 and Balb/c mice had increased upregulation of the SPD-collectin each day after post infection, but the Black 6 relatively had more present. Upregulation of the SPD- collectin was found in the central cornea, peripheral cornea, and conjunctiva of both types of mice. Staining for the SPD-collectin was done to see if this protein may be used in the future as a therapeutic technique to help eyes fight against infection.