Molecular Evaluation of Transgenic Tomatoes from Co-inoculated Cotyledon Pieces
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Gene transfer mediated by non-oncogenic strains of Agrobacterium tumefaciens that contain binary vectors is the method of choice to introduce foreign genes into plants. Commercial use of this technology requires that many "transgenes" eventually be introduced. Large binary vectors are very difficult to construct. As an alternative, small binaries in coinfecting bacteria have been shown to be capable of cotransformation. We were interested in cotransforming tomato plants with two separate Agrobacterium strains each carrying a different binary containing several genes. The DNAs of 30 independent tomato transformants were analyzed by polymerase chain reaction (peR) and Southern blot hybridization. A cotransformation frequency of 20% was observed. This frequency seems to be the product of the frequencies of transformation of the single binaries. In addition copy number of two transgenes that originated from separate coinfecting agrobacteria was not correlated.With honors.