Preliminary Characterizations of Tendon and Spleen Isolates from Chickens Infected with Persistent Avian Reovirus Strain, 1733
Palileo, Ruth Pe
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The avian reovirus strain, 1733, is known to persistently infect gastrocnemius tendons of chickens, causing arthritis. Chickens less than a day old or 1 day old were inoculated with 1733. Viral isolates were obtained from the spleen and the tendon of these chickens at 7, 14, 21 and 49 days post-infection. Chick embryo fibroblasts (CEF) infected with both spleen and tendon isolates evidenced a syncytia-like clumping of cells prior to lysis, behavior not normally observed in the parental strain. This behavior was more marked in CEFs infected with spleen isolates. However, tendon cells infected in the same way evidenced this behavior to a greater extent when infected with tendon isolates. Phenotypic observations were made using immunoflourescent antibody assays. Overall, isolates obtained at a longer time post-infection showed a greater degree of syncytia-like behavior. Preliminary genotypic characterizations were made using polyacrylamide gel electrophoresis for RNA; gels were subsequently silver-stained. Some small migrational shifts occurring in the L2 segment of certain isolates indicate genetic alteration. This data suggests that such syncytia-like clumping of cells prior to lysis results from persistence of strain 1733 in either or both tendon or spleen cells.