In Vitro Affinity Constant Estimation of Two IgG2a Immunoglobulins
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Authors
Shah, Tapan
Issue Date
1991
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Affinity constants were estimated for two radioiodinated
IgG2a monoclonal antibodies against their complementary
antigen receptor cells. Mouse antibodies B 1 and 5G6.4 were
targeted against a lyophilized lymphoma cell line and live
ovarian carcinoma cell line respectively. Radiolabeling by
the iodogen method (Fraker and Speck, 1978) yielded
preparations of 131 I-B 1 and 1251-5G6.4 whose
immunoreactivity was determined by radioimmunoassay plotting
maximal antibody binding as a function of increasing cell
concentration. The affinity constant at equilibrium of each
antibody was then similarly determined by varying the amount
of antibody against a constant number of cells and plotted as
such (Stanley and Lew et aI, 1983). Graphical analysis
showed 131 I-B 1 to have an equilibrium constant of ~1.40 x
10 10 M -1 while 125 I-5G6.4 had a value of ~9.95 x 10 8 M -1.
These data provide a more rigorous assessment of the
radiopharmaceutical quality of radioiodinated monoclonal
antibodies used in clinical studies.
Description
vi, 28 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.