Analysis of Drug Resistance Patterns in 13762NF Rat Mammary Adenocarcinoma Cell Lines
Hathaway, Karen M.
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To study the effects of cellular subpopulation interactions on metastatic stability in polyclonal populations of 13762NF rat mammary adenocarcinoma cell lines, the restriction of clonal diversity was required. This clonal restriction was achieved through drug exposure and selection of drug resistant cell lines (Adr-, DAP-, Oua-, TG- and Vcr-resistant) to act as cell markers. The objective of this research was to identify the patterns of drug resistance of these selected MTF7(T35).3 parent and drug-resistant cell lines using clonogenic colony formation assays to assess survival after 7 d of continuous drug exposure. Dose-response curves were constructed from average percent survival data, 90% lethal dose (LD90) levels were calculated from these dose-response curves, and levels of drug resistance were determined from these values. All cell lines were resistant to their respective selecting agents except the Qua resistant cell line, but the level of resistance varied when compared to the parental F35.3 cell line. Crossresistance was observed primarily for the Vincristine sulfate resistant cell line but to a lesser degree for other cell lines. Individual resistance patterns of the cell lines did not change in coculture indicating no cooperativity between cell lines. These cell lines will be useful for future experiments designed to study the stability of metastatic potential.