Characterization of the Genome of Feline Herpesvirus-1 with Emphasis on the Unique Short Segment
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Authors
Kubas, Cheryl
Issue Date
1988
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Feline herpesvirus-1 (FHV -1) is a herpesvirus of cats
which causes a disease known as Feline Viral Rhinotracheitis (FVR).
The physical characteristics of this disease have been widely described,
but very little work has been done on the viral DNA. In the present
study, The FHV -1 genome was characterized by using restriction
enzymes, primarily Sail, to cut the DNA into smaller and more workable
pieces that were cloned into the plasmids pBR322 and pUC19. Plasmids
were transformed into competent Escherichia coli cells to propagate
enough plasmid to work with and to characterize. The result was a
collection of plasmids that together contained the majority of Sall-cut
FHV -1 fragments. A restriction map was constructed of a plasmid
containing one of the fragments, SaIl B (Rota, Maes, and Ruyechan,
1986). This fragment contains a section of the genome known as the
Unique short (Us), which has been shown in other herpesviruses to
contain glycoprotein genes and other genes that are essential for
virulence, but are not necessary for virus replication. In addition, the
gene coding for thymidine kinase (TK) was searched for by
transforming various SaIl fragment-containing plasmids into L TKcells
which are incapable of salvaging thymidine from medium. No
evidence was obtained to indicate that any fragment of FVR DNA is
capable of transforming L TK- cells to a TK+ phenotype.
Description
vi, 38 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.