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dc.contributor.advisorSternberg, Esther M.
dc.contributor.authorLeone, Nicolo
dc.descriptioniii, 31 p.en_US
dc.description.abstractAn assay was set up to quantify the activity of indoleamine 2,3-dioxgenase (IDO) in human peripheral mononuclear cells(PMC). Interleukin-1 (IL-1), interferon-gamma (IFN-G) and D-ala peptide T (pep-T) were used to stimulate PMC in culture medium. Following a 44 h incubation period, PMC were pulsed with radiolabeled tryptophan in fresh culture medium. After a period of 4 h, PMC supernatants (SN) were aspirated, microfuged, injected into a C18 column and run through reverse-phase high performance liquid chromatography (HPLC) in an attempt to separate tryptophan and its catabolites. Fractions were collected at 30 s intervals and analyzed by means of scintillation spectrometry. Counts per minute (CPM) were plotted as a function of time for each fraction. Percent 100 specific tryptophan catabolism (%SC) was calculated for each biological response modifier (BRM) induced and control run. These data indicated that, although 2 separate HPLC elution systems were used, a satisfactory separation of radiolabeled tryptophan and its catabolites was not achieved. However, compared to the control run for each PMC donor, some tryptophan degradation may have occured in both IL-1 and IFN-G induced PMC, which would indicate IDO activation. Additional chemical analysis or a different HPLC elution method is needed to confirm whether or not IL-1, IFN-G and pep-T induce IDO activation in PMC.en_US
dc.description.sponsorshipNational Institute of Mental Health. Bethesda, Maryland.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleInterleukin-1 Induced Activation of Indoleamine 2,3-Dioxygenase in Human Peripheral Mononuclear Cellsen_US
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  • Biology Senior Individualized Projects [1489]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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