Exploration of the Factors Mediating the Expression of a Retinoblastoma-Associated Antigen Using the Monoclonal Antibody Anti-2-5H:8C
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Authors
Scheffler, Susan A.
Issue Date
1988
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Anti-2-5H:8C is a monoclonal antibody developed against
retinoblastoma, the most common intraocular cancer of childhood.
The 2-5H:C antibody recognizes a retinoblastoma-associated antigen
and binds to each of 3 such cell lines obtained from patients as well
as at least 1 cell line of neuroblastoma. In this study, we attempted
to determine if the expression of the 2-5H:C antigen is cell cycle or
cell type (neuronal vs. glial) dependent. U sing a flow cytometry
technique, the cell cycle dependency of 2-5H:C antigen expression
was ruled out. The 2-5H:C antigen is believed to be expressed only
on tumor cells of primitive neuroectodermal origin' which express
both neuron-specific enolase and glial-fibrillary-acidic protein. The
antigen was also expressed on necrotic cells. The 2-5H;8C antibody
should prove to be useful for diagnostic or therapeutic treatments
when coupled with radiolabeled cytotoxins.
Description
vii, 35 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.