Studies on Tumor Growth Inhibition by Anti-Angiogenic Drugs as Studied on Walker 256 Rat Carcinoma Tumors Grown on the Chorioallantoic Membrane of the Chick Embryo
Burkart, Thomas A.
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An assay for the effect of experimental angiostatic drugs (drugs found to inhibit neovascularization) on growing tumors was developed. Walker 256 rat carcinoma cells were grown in culture and implanted on the chorioallantoic membranes (CAM's) of seven day old chicken embryos. Initial tumor growth and survival studies indicated day seventeen to be the best day to weigh drug treated tumors due to the substantial size of the tumor at this time and the sufficiently high survival rate of the tumor-bearing embryos on this day. Growing tumors were treated on day 8 or 10 of embryo incubation with the angiostatic drugs L-azetidine (LACA), tetrahydrocortisone (THS) and Upjohn's U42129 with and without heparin or Beta-cyclodextrin tetradecasulfate. Embryos were sacrificed on day 17 of incubation, their tumors removed from the CAM and weighed. Although photographic results of certain drug treatments showed a trend in that drug treatment to affect tumor growth, one way analysis of variance of all drug treatment data showed none of the treatments to significantly affect tumor growth. Further studies involving multiple drug treatments, varying drug dosages and how best to apply the drugs as well as other parameters of this assay remain to be done before a conclusion can be drawn as to the effectiveness of this assay in the testing of experimental angiostatic drugs.
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