Microvascular Endothelial Cells and Their Relationship to Angiogenesis
Loading...
Authors
Angeles, Susan M.
Issue Date
1990
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Angiogenesis is the formation of new blood vessels, a process in which endothelial cells
play a major role. In this project, it was determined that endothelial cells, along with a
mixed population of cells, could be harvested from angiogenesis chambers (developed by
Dvorak et aI., 1987) implanted in Sprague Dawley rats. Endothelial cells were evidenced
by their uptake of DiI-Ac-LDL, an endothelial cell marker. It was
found that endothelial cells could be obtained when the extracted fibrin gels were left
whole, resting on a gelatin substrate (to which the endothelial cells could better attach
themselves) in Endothelial Basal Medium supplemented with 10% fetal bovine serum,
antibiotics gentamicin/amphotericin B, and 10 ng/ml basic fibroblast growth factor (bFGF).
However, sustained endothelial cell growth was poor. An isolation procedure for
endothelial cells was performed, but did not yield endothelial cells, demonstrated by the
cells' lack of DiI-Ac-LDL uptake.
In addition, the anti-proliferative effects of suramin, an antitrypanosomal drug (Stein et
ai., 1989), and/or steroids (angiostatic U-24067 and non-angiostatic testosterone) were
examined using two different endothelial cell lines, bovine adrenal capillary (BACE) and
transformed mouse cerebral microvessel (TEn) endothelial cells. Greatest endothelial cell
inhibition was observed with suramin alone and steroid alone (U-24067 or testosterone) at
their highest respective doses tested. Suramin and angiostatic steroid U-24067 did not
yield the expected synergistic inhibition of BACE and TEn cell growth as was shown in the
chorioallantoic membrane (CAM) assay on tumor growth (Wilks et al., 1990). A
synergistic inhibitory effect, however, was observed with suramin and testosterone in this
preliminary experiment. From the dose response experiments, it was concluded that the
anti-proliferative effects of suramin and steroid were non-specific for angiostatic steroids
since no synergistic inhibitory effect was observed with suramin and U -24067 and since
greatest cell inhibition was observed with suramin alone and steroid alone regardless of
whether the steroid was angiostatic or not.
Description
vi, 36 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.