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dc.contributor.advisorZitron, Ian M.
dc.contributor.authorAfsari, Amelia G.
dc.descriptionvii, 64 p.en_US
dc.description.abstractThe CD4 surface antigen on the T cells of the immune system is an important receptor for HIV-1 binding via the glycoprotein 120 (gp120) envelope gene. In this study, a solid phase immunoassay was developed to detect HIV-1 binding to susceptible cells. The HeLa-T4+ cell line, which is susceptible to infection, and the HeLa cell line, which is resistant to infection, were used to develop the immunoassay. Two additional cell lines, SK-N-MC and HepG2, were also used; these lines do not express surface CD4, yet they are susceptible to HIV-1 infection via a CD4 independent mechanism. The immunoassay is performed upon crude membrane fractions dot blotted to nitrocellulose membrane. Immunodetection involved applications with either culture supernatants (A3.0l, a non-producing cell line or 8E5 , a virus producing line) or immunoglobulins (OKT4 or H6a/1). This was followed by a detection system that consisted of incubations with an HIV immunoglobulin standard, biotinylated anti-immunoglobulin antibodies, and enzyme-conjugated streptavidin. Use of a chromogenic substrate indicated the presence of binding. Thus far, the results indicate this immunoassay is effective for cells that express CD4+. Further studies should be completed before we can determine whether the assay has sufficient sensitivity to detect virus binding to CD4 cells.en_US
dc.description.sponsorshipDepartment of Immunology and Microbiology. Medical School. Wayne State University. Detroit, Michigan.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleDevelopment of an Immunoassay for the Solid Phase Deteciton of HIV-1 Binding to Susceptible Cellsen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Individualized Projects [1489]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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