Characterization of a Mutant Form of the glnG Fene Whose Product, Nitrogen Regulator I (NR1), is the transcriptional activator of the glnALG operon responsible for nitrogen assimilation in Escherichia coli
Navai, Yasaman Hannah
MetadataShow full item record
Glutamate is a vital amino acid which serves as a nitrogen donor in the formation of other amino acids and compounds. In conditions of excess nitrogen, such as when ammonia is plentiful, glutamate dehydrogenase provides the primary pathway for glutamate formation. In nitrogen limiting conditions, this enzyme is ineffective in assimilating ammonia. Instead, in enteric bacteria such as Escherichia coli, two different enzymes, glutamine synthetase and glutamate synthase, function in the capacity of nitrogen assimilation and glutamate formation in times of low ammonia. The importance of glutamine synthetase, which is part of the Nitrogen Regulation (Ntr) regulon responsible for nitrogen assimilation, is reflected through its elaborate regulation. GS is encoded by the gInA gene. Transcription of this gene, part of the gInALG operon is activated by the phosphorylated form of Nitrogen Regulator I (NRI) protein (product of the gInG gene). Two compounds, Nitrogen Regulator II (NRII) and acetyl-P, have been demonstrated as donating phosphoryl groups to NRI. This study focused on the characterization of a mutant form of gInG, gInG1128, whose product NRI1128 was believed to activate transcription regardless of its state of phosphorylation. Using a quantitative glutamine synthetase transferase assay in various nitrogen conditions, it was demonstrated that the mutant NRIl128 protein regulated glutamine synthetase transcription less efficiently than did the wild type. Additionally, a second quantitative GS assay revealed that the mutant was not phosphorylation independent, while qualitative assays, demonstrated phosphorylation independence. Examination of both assays and differing growth conditions implicated the possibility of a third compound with the capability of phosphorylating the mutant NRI1128, or even wild type NRI.
Showing items related by title, author, creator and subject.
Root proliferation across a nitrogen fertilizer gradient as a measure of nitrogen use in maize (Zea mays L.) Seehaver, Sarah Ann (Kalamazoo College, 2006)This study investigates corn plant root proliferation in a no till, irrigated maize (Zea mays L.) system by comparing root preference for in growth soil cores with either a control soil or soil homogenized with 2% ground ...
Effects of Soil Nitrogen Abundance on Plant-Rhizobia Mutualisms in Hog Peanut, Amphicarpaea bracteata Foust, Nolan (Kalamazoo, Mich. : Kalamazoo College, 2015)Observe of the effects of N availability on the mutualistic qualities of rhizobia . Measured N availability , plant fitness, and rhizobia fitness.
Effects of elevated nitrogen and fire on the community structure of soil ammonia-oxidizing bacteria in a California grassland Bergh, Kristen (Kalamazoo, Mich. : Kalamazoo College, 2013)Climate change expected to increase rate of soil nitrogen (N) deposition and wildfire frequency in many regions4 Ammonia-oxidizing bacteria (AOB) catalyze the rate-limiting step in nitrification; N2O (nitrous oxide) is ...