A Method for Detection of Newcastle Disease Virus RNA from Double-Crested Cormorant Tissue Samples
Loading...
Authors
Grant, Jill A.
Issue Date
1993
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Newcastle Disease Virus came to our attention when an outbreak occurred
in Double-crested cormorants of the Great Lakes region. This outbreak prompted
our interest in the patterns of viral transmission among cormorant populations as
well as between cormorant flocks and other species. Methods to detect the virus
in infected birds were devised using authentic Newcastle Disease Virus as a
positive control. After isolation of RNA, detection of the virus was possible by
the processes of reverse transcriptase and polymerase chain reaction (RT-PCR).
Determination of the sensitivity of our reactions showed that we were able to
detect 4 genomes of viral RNA using RT-PCR. Analysis by spectrophotometry
indicated that the methods devised resulted in RNA of satisfactory yield and
purity. Further research using these methods would permit early detection of
NDV in samples obtained from field studies. Furthermore, this method would be
valuable in determining possible modes of virus spread between cormorants and
other bird species. The technique will also allow study of RNA sequence
polymorphism among different strains of the virus.
Description
vi, 34 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.