Genetic Characterization of a Yeast Nucleoporin, NUP116
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Authors
Raczniak, Gregory A.
Issue Date
1996
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The nuclear pore complex (NPC) is being studied by several
laboratories, by examining specific proteins located in this structure.
Among these, a family of nucleoporins with an amino acid repeat motif of
glycine-leucine-phenyl alanine-glycine (GLFG) has been the focus of
research in this laboratory. A distinct region found on several of these
nucleoporins (Nup145p, Nupl16p, and Nupl00p) has been shown to bind
homopolymeric RNA in vitro via a novel conserved motif. Deleting
NUPl16 has shown that the gene is not essential but results (nupl16∆) in
temperature sensitivity with slow growth at 23°C. Further studies have
shown that if the carboxyl terminus of Nupl16p (Nup116p-C) is expressed
in the null background, nup116∆, there is no growth, hence, an enhanced
lethality phenotype. The purpose of this study was to investigate the
enhanced lethality phenotype. This phenotype was narrowed down to a
722 base pair region in the carboxyl region of NUP116-C and named
NUP116-CC. Analysis by mutagenesis of NUP116-CC showed possible
areas of interaction by loss of this lethal phenotype when its product is
expressed in a nup116∆ strain. Additional studies of the phenotype by
multicopy suppression are underway to investigate specific interactions of
this region of the nucleoporin.
Description
vii, 39 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.