Testing the Various Efficiencies of Oligonucleotide Synthesis for Use in the Gene Therapy Treatment of Cancer
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Authors
Morehouse, Yasha A.
Issue Date
1996
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
In an effort to produce ever more efficient oligonuleotide
primers for genetic research, a comparison was made between various
types of synthesizing machinery, synthesis methods and purification
methods.
A standard sequence (M13F0R24, see App. A) was run on each of
the four synthesizing machines. The oligonucleotide primer product
was then analyzed in four separate areas:
1) efficiency of synthesis: this information was provided by the
machine itself and was based on the coupling efficiency at each step.
2) yield of the primer: an optical density reading was used to calculate
both the concentration and yield of the product.
3) percent of full length product: chromatography separates the
sample according to size and allows visualization of the amount of
desired length primer.
4) performance in a sequencing application: the primer was used to
sequence of piece of DNA to determine its efficiency in research
applications.
Based on the results obtained in each of these areas, it was
concluded that Beckman 1000 synthesizing ,instruments are superior to
Pharmacia-Biotech in synthesizing oligonucleotide primers.
Description
iii, 42 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.