dc.contributor.advisor | Sun, Frank F. | |
dc.contributor.author | Soles, Stephanie A. | |
dc.date.accessioned | 2011-08-05T12:16:39Z | |
dc.date.available | 2011-08-05T12:16:39Z | |
dc.date.issued | 1993 | |
dc.identifier.uri | http://hdl.handle.net/10920/23107 | |
dc.description | iv, 44 p. | en_US |
dc.description.abstract | When cells are traumatized, arachidonic acid is released from cell
membranes and metabolized into a family of biologically active compounds called
eicosanoids. The origin of these compounds in the brain is uncertain, as is their
role in trauma conditions. Two cell types were studied as possible producers of
eicosanoids: cerebellar granulocytes and astrocytes. Using HPLC to separate
tritiated arachidonic acid metabolites, two compounds--prostaglandin E2
(PG E2) and leukotriene C4 (L TC4)-were found to exist in both cell cultures.
Time-dependent and dose-dependent responses were measured using calcium
ionophore and N-methyl-D-aspartate to stimulate maximum production.
Oxidative stress was simulated in cultures by the addition of various
concentrations of hydrogen peroxide; energy depletion was simulated by treating
cells with sodium iodoacetate. A competitive enzyme immunoassay was used to
quantitate eicosanoid concentrations. Cerebellar granulocyt~s were not able to
produce any measurable amounts of L TC4 while they did produce substantial
levels of PGE2. Rat astrocytes also produced high levels of PGE2; LTC4
production was minimal. When cultures were treated with hydrogen peroxide, it
was discovered that neither PGE2 nor L TC4 synthesis was affected in either
culture; however, the subsequent addition of calcium ionophore caused significant
elevation of PGE2 in both cerebellar granulocytes and astrocytes. Incubation
with sodium iodoacetate resulted in increased prostaglandin levels in both cell
types while L TC4 production remained minimal. These results show that 1.)
neuronal cells are capable of producing PGE2, 2.) PGE2 synthesis is upregulated
during oxidative stress when the release of arachidonic acid is stimulated and 3.)
PGE2 may be involved in stroke as either a participant or as a manifestation of
trauma. | en_US |
dc.description.sponsorship | Upjohn Company. Kalamazoo, Michigan. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_US | en_US |
dc.publisher | Kalamazoo College | en_US |
dc.relation.ispartof | Kalamazoo College Biology Senior Individualized Projects Collection | |
dc.relation.ispartofseries | Senior Individualized Projects. Biology; | |
dc.rights | U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. | |
dc.title | Prostaglandin E2 and Leukotriene C4 Production in rat Cerebellar Granulocytes and Astrocytes: Possible Roles in Brain Trauma | en_US |
dc.type | Thesis | en_US |
KCollege.Access.Contact | If you are not a current Kalamazoo College student, faculty, or staff member, email dspace@kzoo.edu to request access to this thesis. | |