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dc.contributor.advisorCulver, Kenneth W.
dc.contributor.advisorMoorman, Donald W.
dc.contributor.authorKorcek, Andrew Stephen
dc.descriptionv, 41 p.en_US
dc.description.abstractThe murine colorectal carcinoma cell line CT-26, was transduced with the STK retroviral vector (containing the Herpes Simplex virus Thymidine Kinase gene) and tested for its susceptibility to treatment with Ganciclovir (GCV) and for the presence of a bystander effect. The bystander is a phenomenon in which healthy cancer cells die when in the presence of dying cancer cells. After observing a strong susceptibility to GCV and a large bystander effect (40% larger then expected values), a gene therapy treatment was applied to various animal models representing primary, secondary and diffuse tumor burdens. In the primary tumor models, CT -26 cells were injected into the liver of Balb/c mice. After five days STK-Vector producer cells (VPC) were injected into the site of the tumor and GCV treatment was begun. All treatment animals showed significant decrease in tumors volume as compared to the control animals. In the secondary tumor model, CT -26 cells (either containing STK or no vector(NV)) were injected into separate lobes of the "liver. In mice treated with GCV, 89% exhibited complete remission of both STK and NY tumors. This remission is attributed to an immune response mounted by the mouse against the tumor. In the diffuse tumor model, CT-26 NY cells were injected intraperitoneally (Lp.) into the mice and treated with standard STKVPC gene therapy. Mice receiving treatment showed a significant 14 day survival advantage over the non-treatment animals. To better test the in vivo bystander effect, a metastatic model was developed by injecting Cf-26 NY cells into the spleen. The spleens were then either removed or left in the mouse or placed subcutaneously. All models show development of hepatic metastasis, however, only the subcutaneous model prevented widespread peritoneal carcinomatosis before metastases were allowed to form.en_US
dc.description.sponsorshipHuman Gene Therapy Research Institute. Des Moines, Iowa.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleThe Characterization of Murine Colorectal Carcinoma Cell Line CT-26 for Susceptibility to Gene Therapy at a Primary and Secondary Tumor Levelen_US
KCollege.Access.ContactIf you are not a current Kalamazoo College student, faculty, or staff member, email to request access to this thesis.

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  • Biology Senior Individualized Projects [1489]
    This collection includes Senior Individualized Projects (SIP's) completed in the Biology Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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