Determination of the Expression Level of Bax, Bcl-2, and Bcl-X Genes in DBV1, KB31, S49 Deathless and S49 Wild-Type Cell Lines Using Polymerase Chain Reaction
Peracha, Mohammed O.
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Apoptosis or programmed cell death (PCD) is a phenomenon by which cells can be selectively killed through a genetically controlled mechanism. Recently, a relationship between the suppression of apoptosis and cancer development has been proposed. Bcl-2, a gene that suppresses apoptosis, has been found to be over-expressed in some cancer cell lines, and transfection of Bcl-2 in certain cancer cell lines causes them to become resistant to various chemotherapeutic drugs. These facts point to a possible correlation between the drug resistance of certain cancer cell lines and the expression level of apoptosis genes. Two other genes involved in the process of apoptosis are: Bcl-X and Bax. Bcl-X can produce two transcripts: a larger Bcl-XL which suppresses apoptosis and a shorter Bcl-Xs which induces apoptosis. BAX also induces apoptosis. The purpose of this project was to determine the expression level of these genes in drug resistant (KBV1) and drug sensitive (KB31) human KB carcinoma cell lines and in deathless(849 D-) and wild-type(849 wt) mouse 849 lymphoma cell lines, using polymerase chain reaction. 849 D- cell line is resistant to cAMP induced killing while 849 wt is non-resistant. The expression level of three genes was similar in the two KB cell lines. While 849 cell lines showed similar expression level of Bcl-2 and BAX, BcI-XL had a higher expression level in 849 D- cells, where as Bcl-Xs had a higher expression level in 849 wt cells. These results suggest that Bcl-X might play a vital role in the cAMP induced killing pathway.