Effects of Immune Cells on 13762NF Mammary Adenocarcinoma Cell Attachment to Basement Membrane Components
Mantia, Jeffrey S.
MetadataShow full item record
13762NF mammary adenocarcinoma cell clones MTF7 and MTF7(Tl1.2) (Fl1.2) were allowed to adhere to extracellular matrix components. Polymorphonuclear leukocytes (PMN's) were then introduced in various concentrations using several methods to examine the effect of these immune cells on attachment of malignant cells to the basement membrane (BM). Simultaneous PMN and tumor cell addition to matrices and addition after coincubation at several physiological temperatures showed no effect on tumor cell adhesion. PMN's also had no effect on adhesion of MTF7 cells to endothelium. Matrix pretreatment with PMN's followed by a saline wash prior to the addition of malignant cells, however, was shown to have a significant effect on attachment, as the enzymatic action of PMN's facilitated partial liberation of the matrix. It was therefore concluded that PMN's are not involved in the step of the metastatic process involving attachment, but are associated with BM degradation. In addition, studies to determine the cytolytic activity of PMN's and natural killer (NK) cells on tumor cell lines of varying metastatic potential (MTF7, MTF7(Tl1.2), MTC, and MTLn3) were performed, and it was determined that PMN's and NK's do not effectively lyse malignant cells in vitro.