The Detection of a Putative IL-2 Inhibitor and of an IL-2 Facilitator Present in the Synovial Fluid of Patients with Rheumatoid Arthritis
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Authors
Morphew, Emilie
Issue Date
1987
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The synovial fluid of patients with rheumatoid
arthritis was fractionated with ammonium sulfate and
tested for facilitation or inhibition of IL-2 activity.
IL-2 activity was measured by following the
incorporation of tritiated thymidine into a cytotoxic T
cell lymphoma line when cultured with the various
fractions. It was found that facilitation of IL-2
activity by one fraction of the synovial fluid was
apparent. Inhibition of IL-2 activity by a second
fraction was found in isolated instances. One of the
cell lines used, grown in equine serum, was found to be
much more sensitive to IL-2 than the conventional line,
grown in fetal bovine serum. Both lines, when cultured
for two days in IL-2, secreted a factor which inhibited
their own growth.
Description
iv, 39 p.
Citation
Publisher
Kalamazoo College
License
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