A Study of the Combinatory Effects of Recombinant Human Tumor Necrosis Factor and Recombinant Human Interleukin 1 on Lipoprotein Lipase Activity and Lipolysis of 3T3-L1 Cells
Nielsen, Sandra K.
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In this study, the combinatory effects of recombinant human tumor necrosis factor and recombinant human interleukin 1 on lipoprotein lipase activity and lipolysis of 3T3-LI cells were studied. As recombinant human tumor necrosis factor and recombinant human interleukin 1 have been previously demonstrated to individually suppress lipoprotein lipase activity and to stimulate lipolysis of adipocytes, it was hypothesized that the combination of the two monokines would result in an additive or synergistic effect on both LPL activity suppression and lipolytic stimulation. 3T3-LI preadipocytes, a cell line found to be a good model for adipocyte studies, were used. As expected. suppression of lipoprotien lipase activity by the combination of recombinant human tumor necrosis factor and recombinant human interleukin 1 was additive. However, no additive effect on lipolytic stimulation appeared to result from combining the two monokines. Different mechanisms of actions for recombinant human tumor necrosis factor and recombinant human interleukin I on 3T3-LI cells were proposed.
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