The Effect of a Mutation in the Shine-Dalgarno Sequence Preceding the Rop Gene on the Level of Rop Protein Produced
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Authors
Palsrok, Kelli L.
Issue Date
1987
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The exact mechanism by which plasmid replication is
controlled is not yet known. The key elements in plasmid
copy number control have been identified as RNA I, RNA II
and rop. The most widely accepted model hypothesizes that
in order for plasmid replication to occur, RNA II must form
a stable hybrid with the plasmid DNA. By binding to the
RNA II preprimer, RNA I is thought to reduce the initiation
of plasmid replication by inhibiting formation of a
stable RNA II-DNA hybrid. The rop protein is thought to
mediate the formation of the RNA I-RNA II hybrid. It has
previously been determined that if the rop gene is deleted,
copy number increases two- to five-fold . In this paper we examine the effect of a
single base change mutation in the Shine-Dalgarno sequence
of the rap gene on the level of rap produced through the use
of a rop-lacZ gene fusion.
Description
28 p.
Citation
Publisher
Kalamazoo College
License
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